Douglas A. Johnson


Douglas A. Johnson
Adjunct Professor

Room: GNN 256
Office: 613-562-5800 ext. 6347
Work E-mail:

JOHNSON, Douglas A.


Our main interest is trying to answer the questions “How are plant genes regulated” and “what role do insulators play in this process?” Insulators are sequences that can block the effects of enhancers on promoters and/or help to define chromatin regions such as boundaries between euchromatin and heterochromatin. Plant insulators have potential to isolate transgenes from the influence on sequences flanking sites of insertion such as promoters.

Selected publications:
  • Baum, B.R., Edwards, T., Johnson, D.A. Phylogenetic relationships among diploid Aegilops species inferred from 5S rDNA units. Molecular Phylogenetics and Evolution 53: 34-44, 2009
  • Gudynaite-Savitch, L., Johnson, D.A., Miki, B.L. Strategies to Mitigate Transgene-Promoter Interactions. Plant Biotechnology Journal 7: 472-485, 2009
  • Baum, B.R., Johnson, D.A. Molecular confirmation of the genomic constitution of Douglasdeweya (Triticeae: Poaceae) - demonstration of the utility of the 5S rDNA sequence as a tool for haplome identification. Molecular Genetics and Genomics 279: 621-628, 2008
  • Soleimani, V.D., Baum, B.R., Johnson, D.A. Analysis and quantification of retroposon BARE-1 reveals the dynamic nature of the barley genome. Genome 49(4): 389-396, 2006
  • Moïse, J., Han, S.Y., Gudynaite-Savitch, L., Johnson, D.A., Miki, B.L.A. Seed Coats: Structure, Development, Composition and Biotechnology. In Vitro Cellular and Developmental Biology - Plant 41: 620-644, 2005

Fields of Interest

  • Cellular and Molecular Biology
  • Gene Structure and Regulation
  • Insulators
  • Biotechnology
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